Small-Molecule Inhibitors of the m7G-RNA Writer METTL1

TitleSmall-Molecule Inhibitors of the m7G-RNA Writer METTL1
Publication TypeJournal Article
Year of Publication2023
AuthorsNai F., Flores-Espinoza M.P., Invernizzi A., Vargas-Rosales P.A, Bobileva O., Herok M., Caflisch A.
JournalACS Bio & Med Chem Au
Date Published2023 Dec 12
Type of ArticleResearch Article
Keywordsdocking, Drug Discovery, enzymatic assay, epitranscriptomics, m7G writer, MD simulation

We discovered the first inhibitors of the m7G-RNA writer METTL1 by high-throughput docking and an enzymatic assay based on luminescence. Eleven compounds, which belong to three different chemotypes, show inhibitory activity in the range 40-300uM Two adenine derivatives identified by docking have very favorable ligand efficiency of 0.34 and 0.31 kcal/mol per non-hydrogen atom, respectively. Molecular dynamics simulations provide evidence that the inhibitors compete with the binding of the cosubstrate S-adenosylmethionine to METTL1. We also present a soakable crystal form that was used to determine the structure of the complex of METTL1 with sinefungin at a resolution of 1.85A.



Alternate JournalACS Bio. Med. Chem. Au
Highlight Role: 
Drug Design