Fragment-based design of selective nanomolar ligands of the CREBBP bromodomain

TitleFragment-based design of selective nanomolar ligands of the CREBBP bromodomain
Publication TypeJournal Article
Year of Publication2016
AuthorsUnzue A., Xu M., Dong J., Wiedmer L., Spiliotopoulos D., Caflisch A., Nevado C.
JournalJournal of Medicinal Chemistry
Start Page1350
Date Published2015 Jul 15
Type of ArticleResearch Article
Keywordsbromodomains, Drug Design, fragment-based docking, selective inhibitors

Novel ligands of the CREBBP bromodomain were identified by fragment-based docking. The in silico discovered hits have been optimized by chemical synthesis into selective nanomolar compounds, thereby preserving the ligand efficiency. The selectivity for the CREBBP bromodomain over other human bromodomain subfamilies has achieved by a benzoate moiety which was predicted by docking to be involved in favorable electrostatic interactions with the Arg1173 side chain, a prediction that could be verified a posteriori by the high-resolution crystal structure of the CREBBP bromodomain in complex with ligand 6 and also by MD simulations (see Xu , M. ; Unzue , A. ; Dong , J. ; Spiliotopoulos , D. ; Nevado , C. ; Caflisch , A. Discovery of CREBBP bromodomain inhibitors by high-throughput docking and hit optimization guided by molecular dynamics . J. Med. Chem. 2015 , DOI).



Alternate JournalJ. Med. Chem.
PubMed ID26043365
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