Synthesis, radiolabelling and initial biological characterisation of F-labelled xanthine derivatives for PET imaging of Eph receptors

TitleSynthesis, radiolabelling and initial biological characterisation of F-labelled xanthine derivatives for PET imaging of Eph receptors
Publication TypeJournal Article
Year of Publication2020
AuthorsPretze M., Neuber C., Kinski E., Belter B., Köckerling M., Caflisch A., Steinbach J., Pietzsch J., Mamat C.
JournalOrganic & Biomolecular Chemistry
Volume18
Issue16
Pagination3104-3116
Date Published2020 04 29
Type of ArticleResearch Article
ISSN1477-0539
Abstract

Eph receptor tyrosine kinases, particularly EphA2 and EphB4, represent promising candidates for molecular imaging due to their essential role in cancer progression and therapy resistance. Xanthine derivatives were identified to be potent Eph receptor inhibitors with IC50 values in the low nanomolar range (1-40 nm). These compounds occupy the hydrophobic pocket of the ATP-binding site in the kinase domain. Based on lead compound 1, we designed two fluorine-18-labelled receptor tyrosine kinase inhibitors ([18F]2/3) as potential tracers for positron emission tomography (PET). Docking into the ATP-binding site allowed us to find the best position for radiolabelling. The replacement of the methyl group at the uracil residue ([18F]3) rather than the methyl group of the phenoxy moiety ([18F]2) by a fluoropropyl group was predicted to preserve the affinity of the lead compound 1. Herein, we point out a synthesis route to [18F]2 and [18F]3 and the respective tosylate precursors as well as a labelling procedure to insert fluorine-18. After radiolabelling, both radiotracers were obtained in approximately 5% radiochemical yield with high radiochemical purity (>98%) and a molar activity of >10 GBq μmol-1. In line with the docking studies, first cell experiments revealed specific, time-dependent binding and uptake of [18F]3 to EphA2 and EphB4-overexpressing A375 human melanoma cells, whereas [18F]2 did not accumulate at these cells. Since both tracers [18F]3 and [18F]2 are stable in rat blood, the novel radiotracers might be suitable for in vivo molecular imaging of Eph receptors with PET.

DOI10.1039/d0ob00391c
pubindex

0254

Alternate JournalOrg. Biomol. Chem.
PubMed ID32253415