TY - JOUR
KW - Models, Molecular
KW - Protein Binding
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Mutation
KW - Protein Structure, Tertiary
KW - Alleles
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Base Sequence
KW - Chromosomal Proteins, Non-Histone
KW - DNA, Plant
KW - Gene Silencing
KW - Genes, Plant
KW - Histones
KW - Phylogeny
KW - Plants, Genetically Modified
KW - Sequence Homology, Amino Acid
AU - V. Exner
AU - E. Aichinger
AU - H. Shu
AU - T. Wildhaber
AU - P. Alfarano
AU - A. Caflisch
AU - W. Gruissem
AU - C. Köhler
AU - L. Hennig
AB - <p>Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transcriptional repression in animals, but in plants PRC1-like activities have remained elusive. One candidate protein that could be involved in PRC1-like functions in plants is LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), because LHP1 associates with genes marked by H3K27me3 <i>in vivo</i> and has a chromodomain that binds H3K27me3 <i>in vitro</i>. Here, we show that disruption of the chromodomain of <i>Arabidopsis thaliana</i> LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants. Therefore, binding to H3K27me3 is essential for LHP1 protein function.</p>
BT - PLoS One
C1 - 0113
DA - 2009
DO - 10.1371/journal.pone.0005335
IS - 4
J2 - PLoS ONE
LA - eng
M3 - Research Article
N2 - <p>Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transcriptional repression in animals, but in plants PRC1-like activities have remained elusive. One candidate protein that could be involved in PRC1-like functions in plants is LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), because LHP1 associates with genes marked by H3K27me3 <i>in vivo</i> and has a chromodomain that binds H3K27me3 <i>in vitro</i>. Here, we show that disruption of the chromodomain of <i>Arabidopsis thaliana</i> LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants. Therefore, binding to H3K27me3 is essential for LHP1 protein function.</p>
PY - 2009
EP - e5335
T2 - PLoS One
TI - The chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 is essential for H3K27me3 binding and function during Arabidopsis development
VL - 4
SN - 1932-6203
ER -