@article{bibcite_547,
  keywords = {Fluorescence Resonance Energy Transfer, High-Throughput Screening Assays, Enzymes, epitranscriptomics, FRET, enzyme activity, DNA methylation, m6A},
  author = {L. Wiedmer and S. A. Eberle and R. K. Bedi and P. {\'S}led{\'z} and A. Caflisch},
  title = {A reader-based assay for m6A writers and erasers},
  abstract = {<p>We have developed a homogeneous time-resolved fluorescence (HTRF)-based enzyme assay to measure the catalytic activity of N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) methyltransferases and demethylases. The assay detects m<sup>6</sup>A modifications using the natural m<sup>6</sup>A-binding proteins (m<sup>6</sup>A readers). The reaction product or substrate m<sup>6</sup>A-containing RNA and the m<sup>6</sup>A reader protein are fluorescently labeled such that their proximity during binding initiates F{\"o}rster resonance energy transfer (FRET). We show that our HTRF assay can be used for high-throughput screening, which will facilitate the discovery of small-molecule modulators of m<sup>6</sup>A (de)methylases.</p>},
  year = {2019},
  journal = {Analytical Chemistry},
  volume = {91},
  pages = {3078-3084},
  month = {2019 Feb 19},
  issn = {1520-6882},
  doi = {10.1021/acs.analchem.8b05500},
  language = {eng},
}